Journal: Chinese medicine

Article Title: Shen-Bai-Jie-Du decoction suppresses the progression of colorectal adenoma to carcinoma through regulating gut microbiota and short-chain fatty acids.

doi: 10.1186/s13020-024-01019-4

Figure Lengend Snippet: Fig. 9 The schematic diagram of the mechanism of SBJDD. SBJDD may exert its inhibitory effects on colorectal adenoma carcinogenesis by regulating gut microbiota, promoting the production of SCFAs, activating G protein-coupled receptors GPR43, GPR41 and GPR109a, inhibiting histone deacetylases HDAC1 and HDAC3, inducing M2-like macrophage polarization, reducing intestinal inflammation, inhibiting cancer cell proliferation and restoring intestinal barrier function

Article Snippet: Anti-GAPDH (no. 2118S), anti-β-Tubulin (no. 2146S) and Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit (no. 97624 T) were obtained from Cell Signaling Technology (Beverly, USA).

Techniques:

Journal: Neuropsychiatric Disease and Treatment

Article Title: The Impact of Esketamine on Depression: Targeting Oxidative Stress and Neuronal Apoptosis Through BDNF/TrkB/PI3K/AKT Pathway Activation

doi: 10.2147/NDT.S502090

Figure Lengend Snippet: Esketamine Reduces Neuronal Apoptosis and Modulates Apoptotic Pathway Proteins by Activating the BDNF/TrkB/PI3K/AKT Pathway in CUMS Rats. ( A ) Shows the TUNEL staining image for detecting apoptotic cells, magnified at 400×; ( B ) displays the corresponding statistical chart of TUNEL staining, reflecting the relative level of apoptosis in different groups; ( C ) presents the Western blot bands for Caspase-9 and Caspase-3 proteins, illustrating their expression levels; ( D ) shows the statistical chart of the relative expression of Caspase-9 protein, and ( E ) displays the statistical chart of the relative expression of Caspase-3 protein in different experimental groups. *p < 0.05 vs the Normal group, #p < 0.05 vs the CUMS group, and ^p < 0.05 vs the Esketamine group. Results are shown as mean ± SD. N=6. The experiments were performed in triplicate.

Article Snippet: Following that, the sections and apoptotic cells were identified using a TUNEL assay kit (NBP2-31164, Novus Biologicals, USA) as directed by the manufacturer.

Techniques: TUNEL Assay, Staining, Western Blot, Expressing